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BIOLOGY DEPARTMENT > COURSE MATERIALS > VLG > MICROSCOPY > CALIBRATION

Examples for the Calibration of the Ocular Micrometer

Procedure:

Move the stage micrometer until the lines at one end coincide. Then look for another line on the ocular micrometer (arrow) that coincides with one on the stage micrometer. In most instances several stage graduations will be used.

Because the smallest spaces on the stage micrometer equals 0.01 millimeter or 10 um, you can calibrate the ocular micrometer using the following:

Here is an example to give you some practice at calibrating ocular micrometers:

If you wish to see this animation again, click and hold the right mouse button and choose RELOAD FRAME.

Ocular Animation by Chris Knudson (copyright 1999)
Key: Blue = Ocular micrometer
Red = Stage micrometer

In this example, 11 spaces on the ocular (blue) = 7 spaces on the stage (red).
Thus, one ocular space = 7 x .01mm / 11
One ocular space = .0064 mm or 6.4 um

Note: The results (11/7) in the animation are only examples.
Results will differ depending on the ocular micrometer.

For added practice, try these scenarios:

1. 25 spaces on ocular = 7 spaces on the stage
Thus, one ocular space = ?
and one ocular space = ? mm or ? um
Click here for the answer.

2. 17 spaces on the ocular = 11 spaces on the stage
Thus, one ocular space = ?
and one ocular space = ? mm or ? um
Click here for the answer


For comments or questions, e-mail your professor.

 

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