Wartburg Undergraduate
Research Web site:
http://www.wartburg.edu/wcur/
Research/Areas of
Interest
The Occurrence of Antibiotic Resistant Bacteria in Companion Animals
Ecology of
bacterial biofilm communities using molecular methods
Biodegradation
synthetic organic chemicals by Phanerochaete
Biofilm microbial
communities in disease and nature
Antibiotic Resistant Bacteria. Dogs and cats may serve as reservoirs for infections in humans. Current research involves the presence and characterization of antibiotic resistant staphylococci and MRSA in otherwise healthy domestic animals in NE Iowa.
Biofilms. Attached bacterial communities, called biofilms, have
a major impact on ecological, economic, medical, and
industrial processes. We are using molecular methods
(metabolic stains, FISH, DGGE) to study bacterial biofilm
development and activity using CDC bioreactors and flow
cells.
Antibiotic resistant
bacteria in aquatic biofilms. I have been studying
SLIME (microbial biofilms) since my post-doc days at
the University of Calgary. There has been a resurgence
of interest in the spread of antibiotic resistant bacteria
in ecosystems. This project combines the techniques
of microscopy (SEM, FISH) and molecular biology (plasmid
isolation, DGGE) to study antibiotic resistance of bacteria
in epilithic (SLIME ON ROCKS) communities
Analysis of bacterial
communities in Bromeliad tanks. This project
developed from my many trips to the tropics in May term.
Many species of Bromeliads (epiphytic tropical American
plants of the family Bromeliaceae) are structured so
they "trap" water in what are called tanks.
These tank bromeliads contain a myriad of micro- and
macro-organisms. Little has been done to analyze the
bacteria and fungi in these tanks. We are using Scanning
electron (SEM), light, and fluorescent in-situ hydridization
(FISH) microscopy to characterize these communities.
Work is continuing using FISH to determine community
structure.
Biodegradation
of azo dyes by Phanerochaete chrysosporium (PC).
I spent my sabbatical leave in the laboratory of Dr.
John Bumpus at the University of Northern Iowa. Studies
continue on the biodegradative enzymes of PC under various
nutrient conditions. Projects in my lab include growing
PC in biofilms and measuring the effect of nutrients
on enzymes which degrade azo and aryl dyes.