Wartburg Undergraduate
Research Web site:
http://www.wartburg.edu/wcur/
Research/Areas of
Interest
The occurrence of antibiotic resistant bacteria in companion animals.
Isolation and characterization of microorganisms that metabolize antibiotics.
Ecology of microbial communities using molecular methods.
- microbial communities in disease (contact and catheter biofilms, water, soil).
- microbial communities in nature (aquatic ecosystems, soil, sewage treatment, tropical Bromeliads and hypersaline ponds).
Biodegradation of dyes by the fungus, Phanerochaete. (moved from above)
Antibiotic Resistant Bacteria. Dogs and cats may serve as reservoirs for infections in humans. Current research involves the presence and characterization of antibiotic resistant staphylococci and MRSA in otherwise healthy domestic animals in NE Iowa.
Antibiotic resistance and biodegradation in the environment. There has been a resurgence of interest in the spread of antibiotic resistant and antibiotic degrading bacteria in ecosystems. These projects combines the techniques of culturing (plates, tubes, continuous culture, biofilm reactors), microscopy (SEM, DIC and Confocal) and molecular biology (FISH staining, PCR, DGGE) to study antibiotic resistance/degradation.
Biofilms. Attached bacterial communities, called biofilms, have a major impact on ecological, economic, medical, and industrial processes. I have been studying SLIME (microbial biofilms) since my post-doc days at the University of Calgary. We are using culturing and molecular methods (metabolic stains, PCR, FISH, DGGE) to study bacterial biofilm development and activity using CDC bioreactors and flow cells.
Analysis of bacterial communities in Bromeliad tanks and hypersaline ponds. These projects developed from my many trips to the tropics in May term and upcpoming trip to the Bahamas. Many species of Bromeliads (epiphytic tropical American plants of the family Bromeliaceae) are structured so they "trap" water in what are called tanks. These tank bromeliads contain a myriad of micro- and macro-organisms. Little has been done to analyze the bacteria and fungi in these tanks. While much work has been done on microbial ecology of hypersaline systems, there is a dearth of work on the hypersaline ponds in the Bahamas. We are using (SEM), light, and fluorescent in-situ hydridization (FISH) microscopy, PCR and DGGE to characterize these communities.
Biodegradation of azo dyes by Phanerochaete chrysosporium. I spent my sabbatical leave in the laboratory of Dr. John Bumpus at the University of Northern Iowa. Studies continue on the biodegradative enzymes of PC under various nutrient conditions. Projects in my lab include growing PC in biofilms and measuring the effect of nutrients on enzymes which degrade azo and aryl dyes.